FIG. 5.
Feeding by nutritional iron sources. Feeding assays with B. bronchiseptica indicator strains were performed using iron-restricted EDDA agar plates as described in Materials and Methods. The bars indicate the mean diameters of growth stimulation zones, including the 6-mm diameter of the sample well, and the error bars indicate standard deviations (n =3). (A) Growth stimulation by 1.25 mM ferric chloride. (B) Growth stimulation by 300 μM alcaligin. Abbreviations for strains used are as follows: wt, B013N; alcS, BRM16; alcS/alcSBb+, BRM16 (pRK/alcSBp); alcS/alcSBp+, BRM16 (pRKalcSBp).