TABLE 1.
Bordetella strains and plasmids used in this study
| Strain or plasmid | Relevant genotype or description | Reference(s) or source |
|---|---|---|
| B. bronchiseptica strains | ||
| B013N | Wild-type, nalidixic acid-resistant derivative of swine isolate strain B | 4 |
| BRM18 | ΔfauA2 mutant derivative of B013N, with a nonpolar in-frame fauA deletion mutation, alcaligin transport deficient | 18 |
| BRM16 | alcS::Kanr mutant derivative of B013N, with a kanamycin resistance cassette insertion at the unique PstI restriction site in alcS | This study |
| BRM26 | ΔalcA1 mutant derivative of B013N, with a nonpolar in-frame alcA deletion mutation, alcaligin deficient | This study |
| BRM27 | ΔalcA1 alcS::Kanr mutant derived from BRM16 | This study |
| BRM1 | alcA::mini-Tn5 lacZ1 mutant derivative of B013N, alcaligin deficient | 4 |
| BRM30 | ΔalcS1 alcA::mini-Tn5 lacZ1 mutant derived from BRM1 | This study |
| B. pertussis UT25 | Wild-type, virulent phase isolate | 30 |
| Recombinant plasmids | ||
| pBRM6 | 20-kb alcS+ SalI DNA fragment of B. bronchiseptica alcC mutant BRM6 cloned into pGEM3Z, Apr | 4 |
| pBB21 | 2.3-kb alcA+ BamHI-SphI DNA fragment of B. pertussis UT25 cloned into pBBR1MCS, Cmr | Kang and Armstrong, unpublished |
| p3Z/4.6S | 4.6-kb alcABC+ SmaI DNA fragment of B. bronchiseptica B013N cloned into pGEM3Z, Apr | This study |
| pRK/alcSBb | 1.4-kb alcS+ PCR-generated DNA fragment of B. bronchiseptica B013N cloned into pRK415, Tcr | This study |
| pRK/alcSBp | 1.4-kb alcS+ PCR-generated DNA fragment of B. pertussis UT25 cloned into pRK415, Tcr | This study |
| Other plasmids | ||
| pGEM3Z | 2.7-kb cloning vector, Apr | Promega |
| pBluescript II KS(+) | 2.9-kb cloning vector, Apr | Stratagene |
| pRK415 | 10.5-kb broad-host-range cloning vector, Tcr | 42 |
| pBBR1MCS | 4.7-kb broad-host-range cloning vector, Cmr | 3, 43 |
| pEG7 | 8.0-kb allelic exchange vector, Apr Gmr | 26 |
| pEG18.3 | 11.8-kb allelic exchange vector, Apr Gmr Kmr SacBR+ | 26 |
| pBSL86 | 3.6-kb vector source of kanamycin resistance cassette used in construction of alcS::Kanr allele of BRM16 | 1 |