FIG. 2.

Transcription of the abiK operon. Panel A shows the plasmids carried by the six strains used, along with a schematic representation of the cloned insert. The corresponding plasmid copy number in L. lactis MG1363 is indicated by a superscript letter at the end of the plasmid name (see the legend to Fig. 1). The pNZ123 vector was cointroduced as a control in addition to the indicated plasmids in lanes 1 and 2. Panel B presents the Northern blot analysis with a probe corresponding to the abiK gene. To facilitate the comparison of the different constructs, some EOP values from Table 2 (without pSRQ860) are shown above the blot. Bands of the molecular weight marker (in kbp) are indicated on the left.