TABLE 1.

Purifications of rHps-Phi, rHps, and rPhi

Enzyme and step	Activity (U)	Protein (mg)	Sp act (U/mg)	Recovery (%)	Purification (fold)
rHps-Phi
Cell extract	14,400	1,020	14.1	100	1.0
Solubilization	6,580	333	28.8	45.7	2.03
DEAE-Sepharose	3,950	86.5	45.7	27.4	3.24
Solubilization of precipitate formed during dialysis	481	3.12	154	3.3	10.9
rHps
Cell extract	17,100	302	56.7	100	1.0
DEAE-Toyopearl	12,600	78.3	161	73.7	2.84
Butyl-Toyopearl	3,490	14.9	235	20.4	4.14
rPhi
Cell extract	13,300	292	45.5	100	1.0
DEAE-Toyopearl	2,140	12.5	171	16.1	3.76
Butyl-Toyopearl	802	1.48	539	6.04	11.8