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Kinetic parameters of purified rHps-Phi and rHps
| Enzyme | Temp (°C) | Apparent Kma for formaldehyde (mM) | Vmaxa (U/mg) | kcat (s−1) | kcat/Km (mM−1 · s−1) |
|---|---|---|---|---|---|
| rHps-Phi | 60 | 16.9 | 384 | 281 | 45.0 |
| 80 | 6.25 | 526 | 386 | 61.8 | |
| rHps | 60 | 2.31 | 170 | 62.2 | 26.9 |
| 80 | 1.95 | 435 | 159 | 81.5 |
Km and Vmax were obtained by double reciprocal plots of HPS activity versus formaldehyde concentration.
