TABLE 3.
Performance of rapid immunochromatographic test with sera containing various levels of anti-HEV IgG antibodiesa
| Serum no. | HEV ELISA for IgG
|
HEV ELISA for IgM
|
Rapid test IgM score (interpretation) | ||
|---|---|---|---|---|---|
| OD | OD/COV | OD | OD/COV | ||
| 22 | 0.657 | 1.2 | 0.127 | 0.3 | 0 (negative) |
| 6 | 0.673 | 1.2 | 0.028 | 0.1 | 0 (negative) |
| 8 | 0.731 | 1.3 | 0.149 | 0.3 | 0 (negative) |
| 10 | 0.845 | 1.6 | 0.070 | 0.2 | 0 (negative) |
| 9 | 1.669 | 3.1 | 0.005 | 0.0 | 0 (negative) |
| 2 | 3.000 | 5.5 | 0.018 | 0.0 | 0 (negative) |
| 17 | 3.000 | 5.5 | 0.052 | 0.1 | 0 (negative) |
| 35 | 3.000 | 5.5 | 0.583 | 1.3 | 2.5 (positive) |
| 37 | 3.000 | 5.5 | 0.753 | 1.7 | 1 (positive) |
| 41 | 3.000 | 5.5 | 0.786 | 1.8 | 2 (positive) |
| 48 | 3.000 | 5.5 | 0.798 | 1.8 | 2 (positive) |
| 47 | 3.000 | 5.5 | 0.948 | 2.2 | 4 (positive) |
a
Commercial kits from Genelabs Diagnostics (Singapore) were used following the manufacturer's instructions. The COVs for the IgG and IgM test were 0.5 plus the mean OD of the nonreactive control and 0.4 plus the mean OD of the nonreactive control, respectively. A result was considered positive for IgG or IgM anti-HEV only if the OD/COV was equal to or greater than 1.