TABLE 2.
Comparison of hsp-specific IgG titers, presence/absence of active inflammation, score for epithelial chlamydial infection, and site of macrophages labeled by anti-chlamydial IHC in individual koalas grouped by pathology group and titer
| Pathology group and titera | Anti-hsp IgG (log OD)
|
Active inflammationb
|
Scorec for epithelial chlamydial infection
|
IHC-positive macrophages | ||||
|---|---|---|---|---|---|---|---|---|
| Anti-hsp60 | Anti-hsp10 | uFRT | Bladder | lFRT | uFRT | Bladder | ||
| Infected, patent uFRT | −0.64 | −0.76 | + | + | 0.5 | 0 | 2 | |
| −0.28 | −1.48 | + | + | 104 | 41 | 18 | Liver, spleen | |
| −1.17 | −1.42 | − | + | 0 | 0 | 1 | ||
| −0.24 | −1.13 | + | + | 1 | 1 | 2 | ||
| −0.12 | −1.15 | + | + | 65 | 21 | 60 | Exudate, submucosa | |
| Infected, occluded | ||||||||
| Low titers | −1.7 | −1.74 | + | + | 34 | 55 | 17 | Submucosa |
| −0.11 | −1.63 | + | + | 0 | 2 | 3 | Submucosa | |
| −1.85 | −1.33 | + | − | 3 | 3 | 0 | Exudate | |
| −0.3 | −1.15 | − | + | 1 | 0 | 3 | ||
| −0.6 | −1.01 | + | − | 11 | 0 | 0 | Exudate | |
| −0.93 | −0.96 | − | − | 8 | 0 | 0 | ||
| −0.28 | −0.88 | + | + | 5 | 12 | 0 | Submucosa | |
| −0.65 | −0.79 | + | + | 0 | 2 | 0 | Exudate | |
| Mid-range titers (n = 5) | − | + | ||||||
| High titers | 0.1 | −0.76 | − | − | 20 | 0 | 0 | |
| 0.26 | −0.43 | − | − | 1 | 0 | 0 | ||
| −0.06 | 0.01 | − | + | 0 | 0 | 11 | ||
| 0.24 | −0.29 | − | + | 1 | 0 | 12 | ||
| 0.01 | −0.22 | − | − | 0 | 0 | 0 | ||
| 0.19 | 0.06 | − | − | 1 | 0 | 0 | ||
| 0.34 | 0.16 | − | − | 2 | 0 | 0 | ||
Infected indicates positive by PCR or immunohistochemistry. Occluded indicates one or more uteri or uterine tubes occluded by fibrosis. Low titers indicates both anti-hsp60 and hsp 10 titers below the mean. High titers indicates both anti-hsp60 and hsp 10 titers above the mean.
Presence (+) or absence (−) of active inflammation.
Score for chlamydial infection represents the approximate chlamydial load, calculated from the mean number of chlamydial inclusions detected by IHC in each of the components of the uFRT (cervix, ulerus, uterine tubes, ovarian bursa, and ovary), lower FRT (lFRT) (urogenital sinus and vaginal complex), and urinary bladder. The mean numbers of inclusions/cross-section for each component of the FRT were estimated from at least two sections for each side of the FRT.