Skip to main content
. 2024 May 14;25(10):5332. doi: 10.3390/ijms25105332

Figure 2.

Figure 2

Characteristics of ECM proteins produced by young (MmC−) and senescent (MmC+) MSCs: (a) histological identification of collagenous (Sirius Red) and non-collagenous (Fast Green) proteins and their semiquantitative spectrophotometric determination, bar—50 µm; (b) immunocytochemical detection of ECM proteins (ECM proteins—green, DAPI—blue), representative microphotographs, bar—20 µm; (c) the content of pro-collagen 1α1, detected by enzyme immunoassay, in the conditioned medium of young and senescent MSCs; data are presented as M ± SD, p ≤ 0.05, n = 3; (d) relative gene expression (2−ΔΔCt) in MmC+ versus MmC− MSCs encoding ECM proteins: type I collagen (COL1A1), fibronectin (FN1), osteonectin (SPARC), metalloproteinases (MMP1, MMP2), tissue inhibitor of metalloproteinases (TIMP3), and urokinase plasminogen activator (PLAU); data normalized to the expression of HPRT1, RPLP0; * p ≤ 0.05, n ≥ 3.