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Macrophage and granulocyte infiltration in tissues following NAD+ replenishment. (A,C) Representative immunohistochemistry results for F4/80 (A) and Ly6G (C) in heart, liver and lungs harvested from week 15. (B,D) Bar graphs show F4/80 (B) and Ly6G (D) positive numbers (n = 5–8). Scale bar, 100 μm. Data are shown as means ± standard deviations. Differences of three groups were assessed by one-way ANOVA test (B,D). ns, not significant, ** p < 0.01, *** p < 0.001, **** p < 0.0001.
