FIG. 3.

(A) The predicted pseudoknot structure. Nt 55 to 60 in the loop segment of SL-B can interact with nt 112 to 118, excluding the cytosine at nt 116. (B) Site-directed mutations introduced into mut112-118 to examine the base-pairing interactions. (C) Luciferase activity in cells transfected with AV-FL-Luc and mutants as to the predicted pseudoknot structure. Vero cells were electroporated with RNAs, and then at the indicated times after electroporation, cell lysates were prepared and analyzed for luciferase activity. The experiment was repeated at least three times. Standard deviation bars are shown.