FIG. 5.

(A and C) Luciferase activity in cells transfected with AV/EMCV-Luc5′-385 and a series of deletion mutants as to the 5′ UTR. Vero cells were electroporated with the RNAs, and then at the indicated times after electroporation, cell lysates were prepared and analyzed for luciferase activity. The experiment was repeated at least three times. Standard deviation bars are shown. (B) RNA stability of AV-FL-Luc-3Dmut RNA, AV/EMCV-Luc5′-385-3Dmut RNA, and AV/EMCV-Luc5′-111-3Dmut RNA in Vero cell extracts. RNA stability was analyzed as described in the legend to Fig. 2D. Signal intensities of viral RNAs were quantitated, and the percentages of intensities relative to that obtained at 0 h after incubation are shown.