FIG. 2.

(A) Representative colony growth assay after coelectroporation of HCV replicon RNA with nonsilencing (NS) and replicon-specific siRNAs. (B) Histogram of relative percent HCV replicon colony growth after coelectroporation of HCV replicon RNA (T0) or total cellular RNA (T2, T3, T4) with either siRNA 6188 or 6367 versus coelectroporation with nonsilencing control siRNA. The sample numbers represent the serial siRNA treatment numbers. Error bars represent the standard error of three independent experiments. (C) RNA interference activity in T4 HCV replicon cell lines by immunoblot analysis of NS3 and NS5b protein levels after electroporation of siRNA.