FIG. 1.

Products of full-size HCV RNA translation in different cell-free systems. (A) Translation in two preparations of Huh7 S10 (preparation 1, lanes 1 and 2; preparation 2, lanes 3 and 4) and in Krebs-2 S10 (5 and 6). +, present; −, absent. (B) Translation in HeLa S10 (lanes 1 to 3) and in RRL (lanes 4 to 6). The reactions were programmed with HCV RNA in the presence of [³⁵S]methionine at 32°C for 4 h under standard conditions (see Materials and Methods). CMMs (1 μl) were included in the reaction mixtures where indicated. Translation products were separated by SDS-15% PAGE and detected by autoradiography. Analyses of reactions that did not contain mRNA are shown in lanes 1 and 4 of panel B. The positions of the prestained molecular weight protein markers (MBI; Fermentas) are shown at the left. Arrows at the right indicate the major HCV RNA translation products.