FIG. 2.

Genotyping of the UL51 deletion mutant. (A) Agarose gel electrophoresis of PCR products containing the UL51 gene region. BAC plasmid DNA or viral genomic DNA was isolated and analyzed by PCR. Numbers below the gel photos indicate the PCR primer pairs used (Fig. 1B and C). (B) Location of the AscI fragment and schematic diagram of the neighboring loci of UL51. Arrows indicate transcriptional orientations. Thick bar represents the region hybridizing with the probe. (C) Southern blot analysis of AscI-digested DNA isolated from mock-, FDL51-, FDL51R-, YK304-, and HSV-1(F)-infected cells using the EcoRI-HindIII fragment of pBS1007 as a probe (Fig. 1D).