Expression of major tegument proteins. Vero cells were infected with FDL51, FDL51R, YK304, or HSV-1(F) at an MOI of 3. The cells were harvested at 24 h postinfection, and an equivalent amount of protein from each sample was subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis and analyzed by Western blotting with rabbit antiserum against UL51, UL48 (VP16), UL46, or UL49 (VP22) proteins. Molecular mass markers are shown to the left. The arrowheads indicate the position of each protein.