FIG. 2.

(A) Ca²⁺ mobilization analysis in K562 cells transfected with U12 or U51 and treated with IL-8, MCP-1, RANTES, or I-309 and in K562 cells transfected with CXCR1, CCR2, CCR5, or CCR8 and treated with IL-8, MCP-1, RANTES, or I-309. (B) Ca²⁺ mobilization analysis in K562 cells transfected with vector alone, U12, U51, CCR7, or CCR4 and treated with ELC/MIP-3β, SLC, TARC, or MDC. Indo-1 AM-loaded cells were suspended at 2.5 × 10⁶ to 3 × 10⁶/ml and continuously monitored for fluorescence changes. Peptide agonists were added at the time points indicated by the arrowheads at a dilution of 1:25 (vol/vol), resulting in final concentrations of 100 nM. The tracings are from a single experiment that was representative of two separate experiments.