Figure 2.

Evaluation of the distribution of prepared nanoparticles within hepatocytes in vitro and in vivo. (A) Photographs of the DiD-dye labeled synthesized nanoparticles in aqueous solution. (B) Cultured AML12 hepatocytes uptake the nanoparticles labeled with the DiD probe (red). The nucleus was labeled with Hoechst33342 (blue). The images were captured using fluorescence microscope. Scale bar = 10 μm. (C) Cell viability of AML12 hepatocytes incubating with MSN, O@MSN, ON@MSN, and ONL@MSN at the indicated concentration of OCA (0.01–100 μg/mL) for 48 h. (D) A tyramide signal amplification (TSA)-based multiplex-color immunofluorescence staining showing the distribution of DiD dye-labeled nanoparticles in mice liver. The DiD-dye labeled nanoparticles were injected into the tail vein of mice, and the liver tissue of mice was isolated and cut into pieces 24 h later. The TSA multiplex-color immunofluorescence staining was performed and the images were captured using the confocal laser scanning microscope. Albumin (green) indicates hepatocytes. CTNNB1 (orange) indicates the cell membrane. DiD (pink) indicates the nanoparticles absorbed by liver cells. Scale bar = 50 μm.