FIG. 7.

Antibody-mediated CCR5 down-regulation in vivo. The CCR5 phenotype of CD4⁺ cells was evaluated by two-color flow cytometry of cells derived from peripheral blood or from vaginal fluids of mice immunized by mucosal or systemic immunizations with FHV-WT, the synthetic cyclic CCR5_89-102 peptide, or FHV-I2. The double staining was obtained using M20 (anti-mouse CCR5 polyclonal antibody) and GK1.5 (anti-mouse CD4 MAb). (A) CCR5 expression by CD4⁺ cells of mice subjected to mucosal immunizations. The error bars indicate standard deviations. (B) CCR5 expression by CD4⁺ cells of mice subjected to systemic immunizations. (C) Kinetics of CCR5 reappearance on the surfaces of cultured PBMC obtained from mice immunized by either route with FHV-WT or FHV-I2.