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Quantification of 2-LTR circles. 2-LTR circle junctions were quantitated by real-time PCR using 2-LTR circle junction primers in the presence of SYBR green (40 cycles). β-Globin was used as a control for total cellular DNA added to each reaction mixture. Samples were run in triplicate, and the data shown are the means ± 1 standard deviation.
