TABLE 1.
Summary of PPT mutations and results from our previous in vitro assaya
| Mutation | Sequence | Cleavage/extension |
|---|---|---|
| −15+1LTR | ||
| WT PPT | AAAAGAAAAGGGGGGACTGG | ++++/++++ |
| 5′ | GCGCGAAAAGGGGGGACTGG | ++++/++++ |
| 3′ | AAAAGAAAAGGCCCCACTGG | −/− |
| 6Gs | TCATACCATGGGGGGACTGG | ++++/++++ |
| GA | GAGAGAGAGAGAGAGACTGG | +++/++ |
| CG | AAAAGAAAAGGCGCGACTGG | ++/+ |
| GC | AAAAGAAAAGGGCGCACTGG | ++/+ |
| DWN | ACTGGAAGGGCTAATTCACT | −/− |
a
Summary of our previous study (51) using 15- and 20-nt RNA primers containing specific mutations in the PPT sequence annealed to a complementary 35-nt minus-strand DNA template. Changes from the WT PPT sequence (shown in bold) are underlined. The relative amounts of cleavage and extension are represented by + and −. The italic G in the GA oligonucleotide represents an imprecise removal of the RNA primer which results in a base of RNA remaining on the DNA.