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. 2005 Jun;79(11):7024–7041. doi: 10.1128/JVI.79.11.7024-7041.2005

FIG. 10.

FIG. 10.

Inoculation of mice with CVB3/TD results in persistent cardiac virus infections. (A) Total RNA from homogenates of day 175 p.i. hearts of C3H/HeJ mice inoculated with CVB3/TD8 or TD50 was assayed by RT-PCR for the presence of CVB3 RNA with KS1 and KS2. Southern blot analysis (lower panel) with primer E3 confirms that amplified cDNA is CVB3. (B) Heart homogenates were assayed for cellular mRNA with 5-GADPH and 3-GADPH. Southern blot analysis (lower panel) with primer 5-GADPH. (C, D, E) HeLa cells (105) were inoculated with homogenates of day 175 p.i. hearts of C3H/HeJ mice inoculated with CVB3/TD8 (C) or TD50 (D) or with 106 TCID50 units of CVB3/28 (E). Total RNA from 72-h (C, D) or 24-h (E) cultures was assayed by RT-PCR for the presence of CVB3 RNA with KS1 and S, S1, S2, S3, or S4; electrophoresed; and visualized as described in Materials and Methods. Southern blot analysis (lower panels) with primer E3 confirms that amplified cDNA is CVB3. Lane M, 100-bp ladder; lane +, CVB3/28 viral RNA; He, HeLa cell total RNA; lane -, no RNA. The arrow indicates a 600-bp band.