FIG. 2.

Infection of HeLa cells with homogenate of CVB3-infected AJ heart at day 18 does not induce cytopathic effect but does replicate CVB3 RNA. (A) HeLa cultures inoculated with homogenates of hearts at days 14 p.i. (AJ14) and 18 p.i. (AJ18) passed once (P1) and five times (P5) in HeLa as described in Materials and Methods, inoculated with parental virus (CVB3/28) or uninfected cell culture (CC). Cells were stained with crystal violet. (B and C) Total RNA purified from HeLa-passaged AJ14, AJ18, CVB3/28-inoculated HeLa cells, and uninfected cells were used for RT-PCR with the primer 3END and with primers S (B) or E8 (C) (Table 1). The arrow indicates a 3-kb band. Lane M, 1-kb DNA ladder; lanes 1 and 2, AJ14 P1 and 5; lanes 3 and 4, AJ18 P1 and 5; lanes 5 and 6, CVB3/28; lanes 7 and 8, uninfected cell culture. (D) Relative positions in the CVB3 genome of primers used.