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Multiple deletions from the 5′ end exist in cardiac viral populations late in infection. (A) cDNA was dG tailed with TdT, then enzymatically amplified with primers DC-TAIL and E1 (Table 1). Amplimers were cloned and then sequenced using primer E7. Parental CVB3/28 RNA was assayed similarly for comparison. Arrows indicate the 5′ termini of the CVB3/TD genomes. (B) Sequences of 5′ ends of CVB3/28 and CVB3/TD8, -13, -18, -31, and -50.
