FIG. 7.

Effects of A11 repression on proteins involved in early morphogenesis. BS-C-1 cells were infected with 10 PFU per cell of vA11Ri in the presence of the indicated concentrations of IPTG. After 24 h, whole-cell extracts were prepared, fractionated by SDS-PAGE, and analyzed by Western blotting with anti-A11, anti-A17-N (detecting full-length [fl] and proteolytically cleaved [c] forms of A17), anti-pTyr (A17-PY), anti-F10, anti-A14-C (detecting glycosylated [g] and unglycosylated [ug] forms of A14) and anti-P4b/4b antisera. Loading of similar amounts of extract was verified by the detection of similar levels of P4b.