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. 2024 May 10;25(10):5215. doi: 10.3390/ijms25105215

Figure 2.

Figure 2

Leptin promotes VM through Ob-R in human breast cancer cells. VM tube formation assay was performed in MDA-MB-231 cells (A) and Hs 578T cells (B) treated with leptin with and without Ob-R BP. After 16 h of incubation, images were visualized (40× magnification; scale bar = 250 μm) and the number of VM structures formed was counted. Western blot was performed using specific antibodies in MDA-MB-231 cells (C,E) and Hs 578T cells (D,F) treated with leptin with and without Ob-R BP for 30 min (C,D) or 24 h (E,F). Data are reported as the mean ± SD and statistical significance is indicated as *** p < 0.001 vs. untreated control; ### p < 0.001 vs. leptin-treated control.