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. 2024 May 10;25(10):5215. doi: 10.3390/ijms25105215

Figure 3.

Figure 3

Leptin promotes VM through STAT3 signaling in human breast cancer cells. Western blot was performed using specific antibodies in MDA-MB-231 cells (A,E) and Hs 578T cells (B,F) treated with leptin with and without AG490 for 30 min (A,B) or 24 h (E,F). VM tube formation assay was carried out in MDA-MB-231 cells (C) and Hs 578T cells (D) treated with leptin with and without AG490 on a Matrigel-coated well plate. After 16 h of incubation, images were visualized (40× magnification; scale bar = 250 μm) and the number of VM structures formed was counted. Data are reported as the mean ± SD and statistical significance is indicated as *** p < 0.001 vs. untreated control; ### p < 0.001 vs. leptin-treated control.