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. 2024 May 10;25(10):5215. doi: 10.3390/ijms25105215

Figure 6.

Figure 6

AQP1 silencing inhibits leptin-induced VM in human breast cancer cells. Western blot was performed using specific antibodies in AQP1 siRNA-transfected MDA-MB-231 cells (A) and Hs 578T cells (B) treated with leptin for 24 h. VM tube formation assay was carried out in AQP1 siRNA-transfected MDA-MB-231 cells (C) and Hs 578T cells (D) treated with leptin. After 16 h of incubation, images were visualized (40× magnification; scale bar = 250 μm) and the number of VM structures formed was counted. Western blot was performed using specific antibodies in AQP1 siRNA-transfected MDA-MB-231 cells (E) and Hs 578T cells (F) treated with leptin for 24 h. Data are reported as the mean ± SD and statistical significance is indicated as *** p < 0.001 vs. untreated control; ### p < 0.001 vs. control siRNA.