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. 2024 May 9;25(10):5160. doi: 10.3390/ijms25105160

Figure 3.

Figure 3

Differential effect of MTX-211 on PI3K/AKT/mTOR and EGFR signaling pathways in the S1 cell line and ABCG2-overexpressing S1-MI-80 subline. Quantification of relative phosphorylation levels of (A) AKT at T308 (pT308 AKT), (B) S473 (pS473 AKT), and (C) EGFR (pEGFR) in drug-sensitive parental S1 (open circles) and multidrug-resistant S1-MI-80 (closed circles) cancer cells treated with either DMSO (control) or MTX-211 (0–20 μM) for 2 h before being processed for immunoblotting. Human EGF (50 ng/mL) was introduced to the culture medium for 5 min to induce phosphorylation. The values represent the mean ± standard deviation, calculated from more than three independent experiments.