FIG. 5.

Analysis of wild-type and E1^∧E4 mutant organotypic raft cultures. Wild-type and E4M9 organotypic raft cultures were stained with DAPI to determine which cells had positive nuclei. In addition, the rafts were incubated with BrdU for 12 h before being harvested to measure DNA synthesis. Immunofluorescence was performed on cross sections of stratified rafts following incubation with antibodies to BrdU. Cross sections were also analyzed by FISH analysis to determine HPV DNA amplification (open arrows). The closed arrows represent the basal layer.