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. 2005 Jun;79(11):6741–6750. doi: 10.1128/JVI.79.11.6741-6750.2005

FIG. 2.

FIG. 2.

Isotype analysis of specific reactivity in antisera to HPV16 L1 mutants. (A) Reactivity of sera from mice immunized by various HPV16L1 in vitro-assembled preparations in an HPV16 VLP ELISA. Microtiter plates were coated with 100 ng of HPV16 L1 VLP prepared in Sf9 insect cells. Sera of mice (n = 6) immunized with in vitro-assembled preparations of each HPV16 L1 mutant were diluted fourfold and reacted with the plates. Binding of specific antibody analyzed by ELISA using a mouse antibody isotyping kit is shown. (B) Same as above, but the microtiter plates were coated with in vitro-assembled preparations of each HPV16 L1 mutant after affinity purification from E. coli. Representative results from two experiments are shown.