FIG. 3.

Growth of wild-type virus after coinfection with ts mutants. (A) Vero cells were inoculated with tsR, tsS, tsRrev, tsSrev, tsA, tsO, or tsH at an MOI of 5 and simultaneously coinfected with HSV-1 wild type at an MOI of 1.0, while a control culture was singly infected with wt virus at an MOI of 6.0. After 24 h at 39°C, the cells together with the culture media (3 ml) were harvested and analyzed by plaque assays at 39°C. (B) The experimental protocol is identical to that for panel A, with the exception of the use of a 10-fold lower multiplicity of the wt virus in the double infections and of an MOI of 5.1 in the wt single infection. The infectious virus yields (both assayed at 33°C) obtained 24 h after single infections at the permissive (C) or the nonpermissive (D) temperature, respectively, are shown. Values are means of data from three independent experiments; error bars represent standard deviations.