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(A) Particles produced in cells expressing the chimeric G4/φX D protein at 30°C. (B) Particles produced in infected cells expressing the chimeric G4/φX D protein at 37°C. Infectious particles within fractions 1 to 10 (114S virions and 132S provirions) were detected by plaque assays. OD280 readings are presented for fractions 11 onward, which contain uninfectious particles (70S degraded procapsids). Symbols: triangles, wild type; squares, nullD mutant; diamonds, utT204I/nullD mutant.
