FIG. 1.
Identifying the interaction of BPV1 L2 with syntaxin 18. (A) ECTAP BPV1L2 is properly distributed within cells. COS-7 cells were transfected with ECTAP BPV1L2 for 24 h. The left panel shows staining for BPV1 L2, the middle panel shows staining for PML, and the right panel shows a merged image. (B) Coomassie blue-stained gel of TAP extracts from ECTAP BPV1L2-transfected COS-7 cells (lane 1) and ECTAP-transfected control cells (lane 3). Molecular weight markers are shown in lane 2. The proteins labeled 1, 2, and 3 in lane 1 were identified as L2, actin, and syntaxin 18, respectively. No proteins were seen in lane 3. (C) Peptide matches to the syntaxin 18 sequence are shown in bold.