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FIG. 10.

FIG. 10.

Inhibition of HIV-1-env- or SIV-env-mediated cell fusion by rabbit anti-2D7-2SK antibodies. PM1 cells (A) or activated rhesus PBMCs (B) were incubated for 1 h in the absence (PBS) or presence of 10 μg/ml of 2D7 antibody (2D7), rabbit anti-2D7-2SK IgG (affinity purified on peptide column), or control prebleed rabbit IgG, followed by addition of 12E1 cells expressing HIV-1 BaL envelope (A) or expressing SIVmac239 envelope (B). Fusion inhibition was compared to the number of syncytia observed between E/T cells in absence of antibody (PBS) represented as 100%. The numbers of syncytia observed for fusion of 12E1 cells expressing HIV-1 BaL envelope and PM1 cells were 632/well and for the fusion between rhesus PBMCs and 12E1 cells expressing SIVmac239 envelope were 270/well. Data shown are representative of one of three independent experiments. Standard deviations ranged between 5 to 10% of the means for all groups.