FIG. 4.

Biacore sensogram for affinity measurements of MAb 2D7 binding to 2D7-2SK peptide. The sensogram shows MAb 2D7 binding to 2D7-2SK peptide immobilized on a CM5 sensor chip through the free thiol group. Several concentrations of 2D7 antibody were injected simultaneously onto both 2D7-2SK peptide and a third flow cell, which was free of peptide and used as a blank. As a control, anti-ovalbumin antibodies were also injected at the same concentrations on the 2D7-2SK peptide-coupled chip, which showed no binding to this peptide (data not shown).