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. 2005 Jun;79(11):6791–6800. doi: 10.1128/JVI.79.11.6791-6800.2005

FIG. 6.

FIG. 6.

Characterization of rabbit anti-2D7-2SK antibodies. IgG antibodies from rabbit immunized subcutaneously with KLH-conjugated 2D7-2SK peptide were used in peptide and cell ELISA. (A) Serial dilutions of rabbit anti-2D7-2SK antibodies (the starting concentration of antibodies was 283 ng/ml) were added to biotinylated 2D7-2SK captured on streptavidin-coated wells, and the binding was quantified by HRP-conjugated goat anti-rabbit IgG antibody. (B) Rabbit anti-2D7-2SK IgG (28.3 ng/ml) was added to the wells with cultured adherent control Cf2Th canine cells (CCR5−) or Cf2Th cell line expressing CCR5 (CCR5+). Specific antibody binding was measured by HRP-conjugated goat anti-rabbit IgG antibody.