FIG. 8.

Steady-state equilibrium analysis of rabbit IgG binding to 2D7-2SK peptide by Biacore. Various concentrations of rabbit anti-2D7-2SK IgG antibody were injected simultaneously onto both 2D7-2SK peptide immobilized on a CM5 sensor chip through the free thiol group and a third flow cell, which was free of peptide and used as a blank. Binding was recorded using a Biacore 3000 surface plasmon resonance biosensor instrument. As a control, rabbit anti-anthrax PA antibodies were injected at the same concentrations on a 2D7-2SK peptide-coupled chip.