Table 5.
Comparison of 13C NMR chemical shifts in adenine incubated in concentrated sulfuric acid for two different time periods. All NMR data were obtained at room temperature.
| Adenine | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| 13C | ||||||||||
| Solvent | C2 (ppm) | C4 (ppm) | C5 (ppm) | C6 (ppm) | C8 (ppm) | |||||
| 13 October 2022 | 6 November 2023 | 13 October 2022 | 6 November 2023 | 13 October 2022 | 6 November 2023 | 13 October 2022 | 6 November 2023 | 13 October 2022 | 6 November 2023 | |
| 81% D2SO4/19% D2O | 147.92 | 147.90 | 141.78 | 141.76 | 108.61 | 108.60 | 146.67 | 146.67 | 146.11 | 146.15 |
| 88% D2SO4/12% D2O | 148.18 | 148.13 | 142.01 | 141.96 | 108.74 | 108.71 | 146.59 | 146.61 | 145.00 | 145.22 |
| 94% D2SO4/6% D2O | 148.08 | 148.03 | 141.97 | 141.94 | 108.71 | 108.68 | 146.65 | 146.66 | 145.50 | 145.70 |
| 98% D2SO4/2% D2O | 149.55 | 149.47 | 137.37 | 137.78 | 110.22 | 110.14 | 146.43 | 146.45 | 143.42 | 143.35 |