Table 5.
Diuresis and urinary excretion of oxidative stress markers in rats after administration of LDL or oxidized LDL (oxLDL). The rats received intraperitoneal injections of PBS and either LDL or oxLDL at a dose of 4 mg of protein per kg of body weight. Urine samples were collected in metabolic cages 24 h before (pre-injection) and 24 h after (postinjection) the infusion of lipoproteins and PBS.
| Urinary Excretion | Experimental Groups | |||||
|---|---|---|---|---|---|---|
| PBS | LDL | oxLDL | ||||
| Pre-Injection | Postinjection | Pre-Injection | Postinjection | Pre-Injection | Postinjection | |
| Water (mL/24 h) | 8.00 ± 1.00 | 8.00 ± 2.00 | 10.00 ± 1.00 | 12.00 ± 3.00 | 9.00 ± 2.00 | 9.00 ± 1.00 |
| TBARS (µmol/24 h) | 67.00 ± 11.00 | 68.00 ± 6.00 | 97.00 ± 8.00 | 96.00 ± 17.00 | 93.00 ± 14.00 | 93.00 ± 12.00 |
| 8-iso-PGF2α (ng/24 h) | 3.65 ± 1.89 | 3.89 ± 0.98 | 3.39 ± 0.86 | 2.88 ± 0.67 | 3.36 ± 1.57 | 3.00 ± 0.87 |
| 8-OHdG (µg/24 h) | 1.86 ± 0.13 | 1.85 ± 0.28 | 2.19 ± 0.38 | 2.57 ± 0.83 | 1.78 ± 0.33 | 1.82 ± 0.10 |
Abbreviations: TBARS, thiobarbituric-acid-reactive substances; oxLDL, oxidized LDL; 8-iso-PGF2α; 8-isoprostane; 8-OHdG, 8-hydroxy-2′-deoxyguanosine. Results are expressed as means ± standard error of the means (n = 4 for each group).