Table 3.

WGS analysis and SNP identification. This table shows the different SNPs identified in the fluconazole-treated isolates for genes involved in drug resistance, stress response, phenotypic switch, biofilm formation, and immune evasion. Note the sequential accumulation of mutations in the same gene as the drug concentration increases.

		FL-MIC	FL-I	FL-R	FL-2R
Function	Genes	AA Change	AA Change	AA Change	AA Change
Drug Resistance	ERG11	L220L	L220L–L480L	L220L–L480L	L220L–T128K–L264S
	CDR2	I704M–R365Q	I704M–M617L	I704M–M617L	R365Q–I704M–E374K
	MSH2	D73D	T141T	V127	V127A
	CHS3	-	R762P	L321P–D388G–R762P	L321P–D388G–R762P
	ERG4	-	-	-	M157V
Stress response	SOD5	-	A210P	A210P	A210P–G214D
	SOD6	-	S225P	S225P	S225P
	HSP60	K67K	N281N	S267A	S267A
	MNL1	H349R	H349R–I175T	H349R–I175T	H349R–I175T
Phenotypic Switching/dimorphism	SAP1	L53	L53I	L53I	L53I
	CPH1	T510A	T510A	T510A	T510A
	EFG1	K251K	K251K	I384T	I384T
	MFG1	-	A68P	A68P–Q76_Q80del	A68P–Q76_Q80del
	SIR2	L213	L213	L213	L213S
	RFG1	-	-	Q118del	Q118del
	FGR15	V131A	V131A	V131A–S361L	V131A–S361L
Biofilm Formation and Immune Evasion	HWP1	-	-	T296A–S441P	T296A–S441P
	ALS4	I79V–V83I	I79V–V83I–V111A	I79V–V83I–V111A– S186P–D198N	I79V–V83I–V111A– S186P–D198N
	NAG3	-	R158*	Y278H	Y278H
	UME6	A146A	N257S–M267I	N257S–M267I	N257S–M267I –N396S
	SFL1	V691I	V691I	V691I–S373L	V691I–S373L