Table 3.

Primers used for the obtention of 5X and preS amplicons.

Primer Name	Template Specific Primer Positions	Primer Sequence (5′-3′)
Outer M13 PCR
preS_M13_Fw	2822–2843 †	GTTGTAAAACGACGGCCAGTGTCACCATATTCTTGGGAACAA
preS_M13_Rv	57–75	CACAGGAAACAGCTATGACCGAACTGGAGCCACCAGCAG
5X_M13_Fw	1234–1254	GTTGTAAAACGACGGCCAGTATGCGTGGAACCTTTGTGGCT
5X_M13_Rv	1631–1612	CACAGGAAACAGCTATGACCATGGGCGTTCACGGTGGTCT
Nested MID PCR
MID(1-n)_M13_Fw	–	MID(1-n)-GTTGTAAAACGACGGCCAGT
MID(1-n)_M13_Rv	–	MID(1-n)-CACAGGAAACAGCTATGACC

M13, universal adaptor sequence in forward (Fw) and reverse (Rv) primers (underlined sequences). MID(1-n), multiplex identifier sequence, a 10-nt unique identifier for each amplicon from each patient, which allows demultiplexing their reads in a single file. ^† Positions in a genotype A genome; in genotypes B, C, D, E and H, positions 2816–2837; in genotype F positions 2815–2836.