Figure 3.

The levels of migration-related proteins in bladder cancer cells after PSMB4 knockdown. (A) The protein levels of FAK, p-FAK, integrin β1, integrin β3, MLC, and p-MLC in RT4, T24, and J82 cells after treatment with siPSMB4 for 72 h were measured by Western blotting. We used GAPDH as the loading control. (B) The relative quantification of the aforementioned proteins. Data are presented as the mean ± SEM (n = 6). * p < 0.05, ** p < 0.01, and *** p < 0.001 compared to the siRNA negative control group, determined by unpaired t-test with the Mann–Whitney test.