Skip to main content
. 2024 May 24;10(21):eadm8713. doi: 10.1126/sciadv.adm8713

Fig. 2. Repeated production of donor-derived Chinook salmon gametes by dnd-knockout rainbow trout recipients.

Fig. 2.

(A) Restriction fragment length polymorphism (RFLP) analysis of the vasa gene using sperm DNA obtained from 1- to 4-year-old (y/o) trout recipients. Numbers 1 to 10 are sperm samples from trout recipients, CS is Chinook salmon sperm, RT is rainbow trout sperm, U is the uncleaved product of the vasa gene, and C is the restriction enzyme–cleaved product. N indicates negative control without adding templated DNA; M indicates DNA-size marker. (B) Number of sperm produced by 1- to 4-year-old trout recipients. (C) Volume of milt produced by 1- to 4-year-old trout recipients. (D) Number of eggs produced by 2- to 4-year-old trout recipients. (E) Diameter of eggs produced by 2- to 4-year-old trout recipients. Chinook in (B) to (E) indicates number of sperm, milt volume, number of eggs, and egg diameter produced by 4-year-old Chinook salmon, respectively. (F and G) Behavior of donor-derived Chinook salmon germ cells in the gonads of 4-year-old trout recipients was analyzed through H&E staining [(F) and (G)] and immunostaining using the Vasa antibody [(F′) and (G′)]. (F) testis; (G) ovary. Arrowheads indicate spermatogonia [(F) and (F′)] and oogonia [(G) and (G′)]. Scale bars, 20 mm.