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. 2024 May 24;10(21):eadm8713. doi: 10.1126/sciadv.adm8713

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Fig. 4. — (A) RFLP analysis of the vasa gene using sperm DNA obtained from 2- to 5-year-old trout recipients. Numbers 1 to 7 are sperm samples from trout recipients, KS is Kokanee salmon sperm, RT is rainbow trout sperm, U is the uncleaved product of the vasa gene, and C is the restriction enzyme-cleaved product. N indicates negative control in which PCR was performed without adding template DNA; M indicates DNA-size marker. NM indicates not mature; NA indicates not analyzed; D indicates dead. (B) Number of sperm produced by 2- to 5-year-old trout recipients. Kokanee indicates the number of sperm produced by 3-year-old Kokanee salmon. (C) Volume of milt produced by 2- to 5-year-old trout recipients; Kokanee indicates the milt volume produced by 3-year-old Kokanee salmon. (D) Number of eggs produced by 2- to 4-year-old trout recipients. Kokanee indicates the number of eggs produced by a 3-year-old Kokanee salmon. (E) Average diameter of eggs produced by 2- to 4-year-old trout recipients. Kokanee shows the diameter of eggs produced by 3-year-old Kokanee salmon.