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. 2024 Apr 26;60(5):713. doi: 10.3390/medicina60050713

Figure 2.

Figure 2

Phenotypic changes resulting from KLF4 knockdown in polarized macrophages: M0 cells (A–C), M2a cells (D–F), and M2c cells (G–I). (A). Relative expression of KLF4 mRNA in M0 cells. KLF4 was effectively silenced using siRNA. Relative expression of KLF4 mRNA was significantly decreased in the Si compared with the NT (p < 0.05); mean ± SD; n = 3. Statistical analysis was performed using two-tailed Student’s t-test; * p < 0.05. (B). Relative expression of KLF4 protein in KLF4-knockdown M0 cells assessed by Western blotting. (C). Relative expression of MRC1, TNF-α, IL-1β, IL-6, IL-10, and TGF-β mRNA in KLF4-knockdown M0 cells; mean ± SD; n = 3. Statistical analysis was performed using two-tailed Student’s t-test; * p < 0.05, ** p < 0.01, *** p < 0.001. (D). Relative expression of KLF4 mRNA. KLF4 expression in M2a cells was effectively silenced using siRNA. KLF4 expression was significantly decreased in the Si compared with the NT (p < 0.05); mean ± SD; n = 3. Statistical analysis was performed using two-tailed Student’s t-test; * p < 0.05. (E). Relative expression of KLF4 protein in KLF4-knockdown M2a cells assessed by Western blotting. (F). Relative expression of MRC1, TNF-α, IL-1β, IL-6, IL-10, and TGF-β mRNA in KLF4-knockdown M2a (Si M2a) cells. Relative expression of TNF-α mRNA was significantly elevated in the Si M2a compared with the NT M2a cells (p < 0.01); mean ± SD; n = 3. Statistical analysis was performed using two-tailed Student’s t-test; ** p < 0.01. (G). Relative expression of KLF4 mRNA in M2c cells was effectively silenced using siRNA. Relative expression of KLF4 mRNA was significantly decreased in Si M2c cells compared with NT M2c cells (p < 0.001); mean ± SD; n = 3. Statistical analysis was performed using two-tailed Student’s t-test; *** p < 0.001. (H). Relative expression of KLF4 protein in KLF4-knockdown M2c cells assessed by Western blotting. (I). Relative expression of MRC1, TNF-α, IL-1β, IL-6, IL-10, and TGF-β mRNA in KLF4-knockdown M2c cells; mean ± SD; n = 3. Statistical analysis was performed using two-tailed Student’s t-test; * p < 0.05, *** p < 0.001. Ctrl: control, NT: macrophages silenced by non-targeting control siRNA, Si: macrophages silenced by KLF4-siRNA.