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. 2024 May 18;22(5):230. doi: 10.3390/md22050230

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© 2024 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Effects of additives on AlyC7 secretion by three recombinant E. coli strains. (A) Effects of different additives on AlyC7 secretion. Cultures without the addition of any additives were used as controls. (B) Effects of glycine concentrations on AlyC7 secretion. (C) Effects of Triton X-100 concentrations on AlyC7 secretion. The effects of the additives on AlyC7 secretion in (A–C) were analyzed by measuring the extracellular alginolytic activities of the culture supernatants by the DNS method. The data shown in the graphs are from triplicate experiments (mean ± standard deviation [S.D.]).