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. 2024 May 18;22(5):230. doi: 10.3390/md22050230

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© 2024 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Effects of culture conditions on the extracellular production of AlyC7 in strain PelB-AlyC7. (A) effect of the IPTG concentration. (B) Effects of induction time and temperature. (C) The extracellular production of AlyC7 by strain PelB-AlyC7 cultured in the medium containing 500 mM glycine and induced at 20 °C. The effects of culture conditions on the extracellular production of AlyC7 in (A–C) were analyzed by measuring the extracellular alginolytic activities of the culture supernatants by the DNS method. The data shown in the graphs are from triplicate experiments (mean ± standard deviation [S.D.]).