Figure 3.

The phenotype of grain size and quality in FH676 transgene‐free plants. (a) Comparison of plant morphology in BC₂F₂ plants. Scale bars = 20 cm. (b) PCR amplicons of hyg resistance gene in BC₂F₂ and T₂ plants. All positive plants with the hyg‐B‐resistance gene are conducted in T₂ lines. (c–i) Grain morphology (c), brown rice (d), grain length (e), TGW (f), grain yield per plant (g), percentage of chalky grain (h), and chalkiness area (i) in FH676 and BC₂F₂ plants with the heterozygous and homozygous genotypes of Type II. The letters in parentheses represent the same mRNA as OsMADS1 and OsMADS1 ^GW3p6 . Data shown as mean ± s.d. (n = 12 plants). Statistical analyses were performed by Duncan's multiple range tests. The presence of the same lowercase letter denotes a non‐significant difference between means (P > 0.05).