Figure 1.

DR increases survival of 5-FU treated mice.

(A) Scheme of experiments. Young mice (2-month-old) and old mice (20–24-month-old) were exposed to AL diet or DR for 14 days before intraperitoneal 5-FU injection which was daily performed for 5 days, and the diet regimen was continued afterward. For the control group, saline was injected instead of 5-FU.

(B,C) Survival was monitored daily after 5-FU treatment (Data combined from 3 independent experiments. n = 30 mice per group for the AL + 5-FU group and DR + 5-FU group; n = 10 mice per group for the young AL+Saline group and young DR+Saline group; n = 6 mice per group for the old AL+Saline group and old DR+Saline group).

(D,E) Percent change of body weight at indicated timepoints after 5-FU treatment compared to before 5-FU treatment (n = 30 mice per group. Data combined from 3 independent experiments).

(B,C) Gehan-Breslow-Wilcoxon test; (D,E) Unpaired two-tailed, Student’s t test. Results were displayed as mean±SD. **p < .01; **** p < .0001. YAL+Saline: young mice on AL diet and received saline injection; YDR+Saline: young mice on DR diet and received saline injection; YAL + 5-FU: young mice on AL diet and received 5-FU injection; YDR + 5-FU: young mice on DR diet and received 5-FU injection. OAL+Saline: old mice on AL diet and received saline injection; ODR+Saline: old mice on DR diet and received saline injection; OAL + 5-FU: old mice on AL diet and received 5-FU injection; ODR + 5-FU: old mice on DR diet and received 5-FU injection; d1: day 1 after 5-FU injection; d3: day 3 after 5-FU injection.