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. 2024 May 23;16(1):2355693. doi: 10.1080/19490976.2024.2355693

Figure 4.

Figure 4.

Abx administration rescued survival of AL mice after 5-FU treatment.

(A) Scheme of experiments. Young mice (2-month-old) and old mice (20–24-month-old) were exposed to AL diet or DR for 14 days before intraperitoneal 5-FU injection which was daily performed for 5 days (day-4–day0), and the diet regimen was continued afterward. Seven days (day-10) prior to 5-FU administration, mice were given a gavage of broad-spectrum antibiotics (Abx group) or saline (control group) for 5 days and then in drinking water for the following days.

(B,C) Survival was monitored daily after 5-FU treatment (Data combined from 2 independent experiments. n = 10 mice per group for the young AL + 5-FU group and DR + 5-FU group; n = 25 mice per group for the young AL+Abx +5-FU group and DR+Abx +5-FU group; n = 20 mice per group for the old AL + 5-FU group, DR + 5-FU group, AL+Abx +5-FU group, and DR+Abx +5-FU group).

(D) Representative images of H&E staining of jejunum on day 3 after 5-FU treatment from indicated groups. Scale bar: 50 μm.

(E-G) Villi frequencies, villi height, and VH/CD (the ratio of villi height versus crypt depth) (n = 5 mice per group randomly picked from 2 independent experiments).

(H) Representative images of Immunohistochemistry staining of Olfm4 of ileum on day 3 after 5-FU treatment from indicated groups. Scale bar: 20 μm.

(I) Basal crypt Olfm4-positive cell number per crypt on day 3 after 5-FU treatment (n = 5 mice per group randomly picked from 2 independent experiments).

(B,C) Gehan-Breslow-Wilcoxon test; (E-G, I) One-way ANOVA test. Results were displayed as mean±SD. *p < .05; **p < .01;***p < .001; ****p < 0.0001; ns, not significant. YAL+Saline: young mice on AL diet and received saline injection; YDR+Saline: young mice on DR diet and received saline injection; YAL + 5-FU: young mice on AL diet and received 5-FU injection; YDR + 5-FU: young mice on DR diet and received 5-FU injection;YAL+Abx +5-FU: young mice on AL diet and received a gavage of Abx and 5-FU injection; YDR+Abx +5-FU: young mice on DR diet and received a gavage of Abx and 5-FU injection. d3: day3 after 5-FU injection; Abx: broad-spectrum antibiotics.